"Isolation and characterization of endophytic bacterium Bacillus cereus in detoxification of Mycotoxin-Aflatoxin B1"

Authors

Dr. Manjunatha A S
Ramaiah College of Arts, Science and Commerce, Bengaluru-560054

Abstract

Aflatoxinsbring great significant cause to food, feed industry and detrimental effects on humans including carcinogenic, mutagenic and teratogenic. The research mainly focuses on efficiency of Bacillus sp. Strain AIGN8 to degrade aflatoxin B1 concentration of (2μg/mL) as a sole carbon source isolated endophytic bacteria from Neem (Azardirachtaindica) belonging to family Meliaceae. The residual AFB1 determined by chromatographic methods. Bacillus sp. Strain AIGN8 had strong ability to degrade the highly toxic AFB1, of 68.9% by culture supernatant compared cell extracts and viable cells at 48hrs of incubation. Moreover, the results implied that the activity of aflatoxin B1 detoxification was significantly noticed in the cell supernatant rather than cell extract and viable cell culture. The cell supernatant was characterized by considerable activity at wide range of temperature and pH.AflatoxinB1 degraded products were identified by GCMS, the compound BETA-MONOSTEARINE with retention time 38.844 of molecular weight 358.56 Da is the major degradative compound with good biological properties. The morphological, biochemical and 16S rRNA sequencing of the isolate AIGN8revealed that belongs to Bacillus cereus and nucleotide sequence was provided with a GenBank accession number MG957213. Biosafety results indicated that AFB1 detoxification is an enzymatic and could have promising potential in industrial applications. First time we reported endophytic bacterium Bacillus cereus in the degradation of AFB1by this research work.